Includes

Materials for six separations: ion exchanger, CM-Cellulose; concentrated Potassium Acetate buffer, pH 6.0; dye mixture: blue dye, Cytochrome c; Cytochrome c Standard (10mg/mL); chromatography columns; plastic pipets; 1mL pipet; 15mL conical tubes; microcentrifuge tubes; complete instructions; background information; and study questions

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Requires

5mL test tubes; ring stand with clamps for columns; 100 or 200mL beakers or flasks; 100, 250 and 500mL graduated cylinders; distilled water; 5mL pipets and pumps; and spectrophotometer and cuvets (optional, must be able to read 1mL samples)

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Learn the principles of ion-exchange chromatography.

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